
AI2 Incubator is coming into its subsequent section.
The Seattle-based startup group — identified for spinning out firms on the intersection of AI and real-world functions — has closed an $80 million third fund to help about 70 new tech ventures over the following 4 years.
GeekWire first reported the fund in July. This week, we sat down with Jacob Colker, managing director of the 10-year-old incubator, to debate classes from a decade of AI startup constructing and the way the agency is positioning itself for the following wave of entrepreneurship whereas serving to enhance Seattle’s tech ecosystem within the international AI increase.
Classes realized and a brand new chapter
Inside Colker’s workplace on the incubator’s new waterfront headquarters, a wall of framed founder photographs is a visible reminder of the group’s observe file — from alumni reminiscent of pc imaginative and prescient firm Xnor.ai (acquired by Apple) and contract administration software program agency Lexion (acquired by Docusign) to newer startups together with Ozette (biotech knowledge), Yoodli (AI roleplay teaching), Vercept (automated desktop workflows), and Casium (immigration processing).
Of greater than 50 firms that graduated from AI2 Incubator, practically 1 / 4 have been acquired and 90% went on to boost enterprise funding.
Founders who’ve gone by means of this system credit score it for serving to supercharge progress.
“Total, they have been an enormous accelerant for Lexion, giving us the expertise, AI recommendation, and credibility to land a strong group, nice buyers, and clients,” stated Lexion co-founder Gaurav Oberoi, whose firm bought for $165 million final yr.
The brand new fund comes amid each record investor enthusiasm for AI startups and rising scrutiny over which ventures have actual depth past the present hype. There may be additionally stiff competitors from numerous different enterprise corporations, accelerators, startup studios, and incubators seeking to discover the following nice AI firms.
Colker stated Fund III displays classes realized from the incubator’s first two funding cycles.
“We backed some developer instruments and infrastructure performs, and we additionally supported a number of groups that have been commercially robust however didn’t have deep technical foundations,” he stated. “Each have been helpful experiments, however in hindsight, a few of these firms plateaued.”
Developer-focused startups confronted fierce competitors and lengthy gross sales cycles, he stated, whereas execution-first groups with out deep AI experience struggled to construct actual differentiation or turn out to be enticing acquisition targets.
With its third fund, the incubator is doubling down on its well-worn technique of pairing vertical, real-world functions with strong technical understanding.
“Each one in all these firms has someone on the group who actually is aware of the trade that they’re going into, and actually understands the issues,” Colker stated.

AI2 Incubator prides itself on AI experience, rooted in its origins throughout the Allen Institute for AI (Ai2), the Seattle nonprofit analysis institute based by Microsoft co-founder Paul Allen.
“We began doing utilized AI commercialization in 2015 — again then, it was largely theoretical,” Colker stated. “And now it’s the centerpiece of the innovation sector, in loads of methods.”
In Colker’s view, the panorama for AI founders has shifted dramatically because the incubator’s early days. He described what he calls the “subsequent chapter of entrepreneurship within the age of AI”: startups with specialised, vertical options led by founders with years of area data — individuals who perceive the issues inside hospitals, factories, and monetary programs, and are actually pairing that data with AI know-how.
Colker stated it’s getting more durable to seek out clear sign within the “AI fog” as the road between novelty and actual technical depth blurs. “An API with a coat of paint on it — that’s not an AI firm,” he stated.
AI2 Incubator, which invests throughout numerous industries, pays shut consideration to an organization’s knowledge entry benefits, specialised distribution channels, workflow integration, and buyer belief.
“If we will’t see a transparent hyperlink between the technical perception and enterprise worth, we don’t have interaction,” Colker stated.
Flexibility and focus
AI2 Incubator usually works with founders on the earliest stage, typically even earlier than they’ve integrated an organization.
It invests as much as $600,000 in every startup through a SAFE (Easy Settlement for Future Fairness) with a $10 million valuation cap and usually holds about 7% in frequent shares — the identical class as founders, somewhat than most well-liked fairness.
The fairness stake is in the identical ballpark as Y Combinator, and decrease than some studios and incubators that will take a extra hands-on method to constructing firms.
AI2 Incubator additionally offers as a lot as $1 million in cloud computing credit and entry to its technical and analysis community, together with AI specialists from firms reminiscent of Microsoft and Amazon.
“What makes the AI2 Incubator particular is the depth of in-house experience,” stated Priyanka Kulkarni, CEO at Casium.
Colker stated Fund III was considerably oversubscribed. AI2 Incubator works carefully with about 15 firms per yr.
“Staying small isn’t a limitation; it’s our benefit,” he stated.
In contrast to accelerators that transfer startups by means of mounted cohorts, AI2 Incubator accepts firms on a rolling foundation. There are additionally no required board seats or relocation mandates. Founders can apply year-round and work both in Seattle or remotely.
The mannequin, Colker stated, is designed for flexibility — to draw skilled entrepreneurs who might not match the life-style of a three-month accelerator dash.
Seattle’s AI alternative

Seattle stays the house base for AI2 Incubator, although 30% of its founders should not within the metropolis. Colker expects that share to succeed in 50% with the brand new fund.
AI2 Incubator firms are required to spend no less than per week per quarter in Seattle, and a few relocate or transfer a part of their workforce to the town.
Colker leads the incubator with fellow managing director Yifan Zhang, a longtime entrepreneur who spearheaded AI House, the sprawling new startup constructing at Pier 70 alongside Seattle’s waterfront that serves as AI2 Incubator’s headquarters together with occasion house and co-working workplaces.
Since its launch in March, greater than 15,000 individuals have handed by means of its doorways, in accordance with the incubator, drawn by a mixture of technical deep dives and startup programming.
AI Home, supported partially by metropolis and state authorities funding, is a part of a broader effort to spice up Seattle as a global AI hub.
Colker stated the area’s strengths — deep technical expertise, proximity to Microsoft and Amazon, and a collaborative analysis tradition — give it an edge in constructing sturdy AI startups that clear up actual enterprise issues.
However he acknowledged that the Bay Space does have an edge in group round entrepreneurship. “You’ll be able to’t stroll down the streets of Palo Alto with out listening to 15 startup pitches,” he quipped.
New efforts like AI Home and Foundations will assist fill that void in Seattle, Colker stated.
“The cloud was invented right here. The expertise is right here. The range of industries are right here. The standard of life is right here,” he stated. “There’s now funding right here, group right here — it’s actually, unbelievably properly positioned.”
He added: “There’s simply no excuses anymore as a result of we now have every thing we’d like. I believe we’re going to get there, and we’re going to proceed to develop.”
Develop and thrive

AI2 Incubator’s third fund represents a bounce from the $30 million raised for Fund II in 2023 — although it’s unfold over 4 years.
The brand new fund drew backing from Khosla Ventures, Point72 Ventures, and Madrona Enterprise Group, together with company buyers together with BHP Ventures and SBI Group.
Tim Porter, managing director at Madrona, cited the rising nationwide attain of the AI2 Incubator program and fame as “a spot the place nice AI founders can come to succeed.”
AI2 Incubator spun out from the Allen Institute for AI after Allen’s loss of life in 2018, changing into an impartial group with its personal governance and funding construction.
The Allen Institute for AI maintains a “small share of non-governing possession” within the incubator, Colker stated.
Colker stated there are nonetheless a few dozen “resident specialists” at AI Home that each work on the analysis institute and spend time with founders on the incubator.
AI2 Incubator can also be led by technical administrators Oren Etzioni and Vu Ha, who proceed to information founders on product improvement and technical technique.
Etzioni, who helped launch and beforehand ran the Allen Institute for AI, instructed GeekWire that the incubator was a part of the unique conception of Ai2 again in late 2013.
“We spun it out of the nonprofit institute to allow it to develop and thrive,” he stated. “Now, with Fund III, we’re poised to indicate the world what decided founders can accomplish amid the AI increase.”